Environmental Thesaurus

Last uploaded: September 14, 2024
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ID

http://vocabs.lter-europe.net/EnvThes/20028

Preferred Name

enzyme assay

Definitions

[MESH] Methods used to measure the relative activity of a specific enzyme or its concentration in solution. Typically an enzyme substrate is added to a buffer solution containing enzyme and the rate of conversion of substrate to product is measured under controlled conditions. Many classical enzymatic assay methods involve the use of synthetic colorimetric substrates and measuring the reaction rates using a spectrophotometer. Sensitive detection methods using enzymes to indirectly measure substances that would normally be difficult to quantitate.

In Schemes
Type

http://www.w3.org/2004/02/skos/core#Concept

creator

herbert.schentz@umweltbundesamt.at

definition

[MESH] Methods used to measure the relative activity of a specific enzyme or its concentration in solution. Typically an enzyme substrate is added to a buffer solution containing enzyme and the rate of conversion of substrate to product is measured under controlled conditions. Many classical enzymatic assay methods involve the use of synthetic colorimetric substrates and measuring the reaction rates using a spectrophotometer. Sensitive detection methods using enzymes to indirectly measure substances that would normally be difficult to quantitate.

prefLabel

enzyme assay

created

2016-03-31

broader

http://vocabs.lter-europe.net/EnvThes/20138

modified

2018-07-02

exactMatch

http://purl.bioontology.org/ontology/MESH/D057075

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